VidaCode TM Nucleotide Puzzle Pieces Used to Model Events during Polymerase Chain Reaction (PCR) and Sanger Sequencing of DNA

 

Tested Studies in Laboratory Teaching, 2018, Volume 39

Valerie Schawaroch, Mary G. Egan, & Elizabeth Torres

Abstract

Learning is enhanced when more than one sensory modality is used. Here we present VidaCodeTM 3-D puzzle pieces that represent DNA nucleotides and dideoxy nucleotides, and exercises that use visual and kinesthetic senses to teach Polymerase Chain Reaction and Sanger sequencing for high school or college biology students. Each nucleotide puzzle piece has a unique shape and color. Initially, students self-guide assembly of puzzle pieces to discover features of DNA structure. Student groups then build primers and a double-stranded DNA molecule and use them to model the events that occur in the first three cycles of PCR and in Sanger sequencing. For sequencing, groups of students collaborate and class results are pooled to complete the model. Detailed instructions are provided and include embedded questions for assessment. VidaCodeTM also can be used to improve understanding of DNA replication, additional sequencing technologies, genotyping, restriction enzymes, primer design, and DNA cloning.

Keywords:  PCR, DNA, visual learning, kinesthetic learning, puzzle, Sanger sequencing, VidaCode TM

University of Wisconsin, Madison (2017)